Wrap Seurat Multiomics clustering — Multi_Wrapper • redeemR

This function allows you to perform standard sc-multiome clustering

Usage

Multi_Wrapper(
  path =
    "/lab/solexa_weissman/cweng/Projects/MitoTracing_Velocity/SecondaryAnalysis/Donor01_CD34_1_Multiomekit/CellRanger/Donor01_CD34_1/outs",
  atacmin = 1000,
  umimin = 1000,
  CellID = NULL
)

Arguments

path: this should be the path to the cell-ranger results XX/outs
atacmin: minimum atac fragment for each cell, default is 1000
umimin: minimum rna umi for each cell, default is 1000
cellID: to be used for input(useful for re-clustering), default is NULL which will use the info from path/per_barcode_metrics.csv

Value

this returns seurat object with both RNA and ATAC

Examples

if (FALSE) {
Multi_Wrapper(path = "XX/CellRanger/Donor01_BMMC_1/outs/")
}